VAV1 Antikörper (pTyr174)

Produktnummer ABIN7469173

Der Kaninchen Polyklonal Anti-VAV1 Antikörper wurde für WB validiert. Er ist geeignet, VAV1 in Proben von Human zu detektieren.

Kurzübersicht für VAV1 Antikörper (pTyr174) (ABIN7469173)

Target: VAV1 (Vav 1 Oncogene (VAV1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser VAV1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-VAV1 Antikörper

Bindungsspezifität: pTyr174

Kreuzreaktivität: Human

Aufreinigung: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide surrounding phospho Tyr174 of human VAV1. The exact sequence is proprietary.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Kommentare:

Positive Control: Jurkat (serum starvation for 15 min then 0.13 μg/mL anti-T-cell receptor antibody (clone 305) treatment for 5 min) Validation: Orthogonal

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.23 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Details zu VAV1

Target: VAV1 (Vav 1 Oncogene (VAV1))

Andere Bezeichnung: vav guanine nucleotide exchange factor 1

Hintergrund:

Synonyms: vav guanine nucleotide exchange factor 1 , VAV

Background: The protein encoded by this proto-oncogene is a member of the Dbl family of guanine nucleotide exchange factors (GEF) for the Rho family of GTP binding proteins. The protein is important in hematopoiesis, playing a role in T-cell and B-cell development and activation. This particular GEF has been identified as the specific binding partner of Nef proteins from HIV-1. Coexpression and binding of these partners initiates profound morphological changes, cytoskeletal rearrangements and the JNK/SAPK signaling cascade, leading to increased levels of viral transcription and replication. [provided by RefSeq]

Molekulargewicht: 98 kDa

Gen-ID: 7409

UniProt: P15498

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, CXCR4-mediated Signaling Events, BCR Signaling